Immunoassay Development Services
Immunoassay Development Services
Customized Sandwich-Based Immunoassay Design
- We specialize in designing sandwich-based immunoassays tailored to detect your specific targets of interest, ensuring precise and reliable results.
Our assays are compatible with both, conventional ELISA platforms and AVAC –our cutting-edge proprietary technology– giving you flexible detection options.
Customized Sandwich-Based Immunoassay Design
Mecwins designs immunoassay technologies based on customer molecules, including proteins, nucleic acids, antibodies, and small molecules. We develop and optimize diverse surface chemistries compatible with biomolecules to enhance assay performance.
Protein Immobilization Services
- We provide expert services in attaching antibodies to surfaces, achieving highly dense and oriented immobilization, maximizing the effectiveness of your assay.
Our team also specializes in attaching unique tags to antibodies, reducing non-specific binding and improving the accuracy of your results.
- Immobilization can be applied across a wide range of materials, providing versatility and customization based on your project requirements.
Protein Immobilization Services
Mecwins has considerable expertise in the immobilization of antibodies of any format, including full-length antibodies, Fabs, and nanobodies, onto an array of surfaces in accordance with the specifications delineated by our customers. Furthermore, our capabilities extend to the attachment of unique tags to antibodies and the development of surface chemistries compatible with them, which facilitate the high orientation of the molecules on the surfaces and, consequently, enhance sensitivity.
Antibody Affinity and Avidity Testing Services
- We assess the strength of interactions between antibodies and their specific target epitope to help you select the best antibodies for your specific assays, ensuring high sensitivity and specificity.
Antibody Affinity and Avidity Testing Services
We have conducted KD (equilibrium dissociation constant between antibody and antigen) measurements for over 15 monoclonal antibodies (mAb) from one of our key customers. KD is defined as the ratio of the dissociation rate constant (kon) to the association rate constant (koff) and it represents the equilibrium between an antibody and its antigen. KD and affinity are inversely proportional, i.e. lower KD values indicate a higher antibody affinity. To achieve ultrasensitivity, it is recommended to use high-affinity antibodies with KD values in the picomole to femtomole range.
Antibody Pair Screening Services
- We help identify the most effective antibody pairs for capturing and detecting your target antigens, ensuring optimal performance of your immunoassay.
- Our screening process evaluates key parameters such as specificity, sensitivity, and background noise, providing you with accurate and dependable results.
Antibody Pair Screening Services
We are pleased to offer our customers the option of sandwich pair screening for their molecules. This process entails testing all antibodies as both capture and detection antibodies in all possible combinations to identify the most suitable sandwich pairs. The detection antibodies are correctly identified and labelled (either biotinylated or conjugated to HRP for ELISA assays). An ELISA positive pair is defined as an antibody that produces a specific signal five times above background (as indicated by the green cells in the table). Antibodies that meet the desired specificity requirements are selected for the customer. Additionally, to maximize the potential of your molecules and to improve the sensitivity of your assay (up to an order of magnitude in LLoD), we can develop kits using your antibodies together with our ultra-sensitive AVAC proprietary technology.
Reagent Selection and Validation
- Our experts assist in selecting or designing the reagents (e.g., diluents, buffers) that are most appropriate for your specific immunoassay, ensuring robust performance.
- We also provide feasibility assessments for proprietary or commercially sourced reagents, giving you confidence in selecting the best candidates to move forward with in your development process.
Reagent Selection and Validation
Mecwins assists its clients in identifying the optimal assay diluent formulation for their specific assays. We optimize the components to minimize matrix interferences observed in standard immunoassays and to achieve an acceptable percentage of recovery in real samples.
Product Testing Services
- We conduct comprehensive feasibility studies on your products using a variety of human biofluids, helping you validate the performance and reliability of your diagnostic tests in real-world conditions.
Product Testing Services
Mecwins has extensive experience in selecting the most appropriate matrices for each assay, analyzing the most common interferences of each matrix for each particular assay. Mecwins will help you optimize the diluent assay to get the best performance for each matrix.
